Study on possible utilization of metal oxide based stationary phases for the analysis of polar compounds

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Student: Jitka Slaninová Supervisor: Doc. PharmDr. Petra Kovaříková, Ph.D. Title of diploma thesis: Study on possible utilization of metal oxide based stationary phases for the analysis of polar compounds This diploma thesis is aimed at investigation of the ability of zirconia (column ZirChrom®-PHASE, 150 x 4,6 mm; 5 µm) and titania (column Sachtopore®-NP, 150 x 4,6 mm; 5 µm) based stationary phases for analyses under the conditions of hydrophilic interaction chromatography (HILIC). Influence of mobile phase strength, mobile phase pH and buffer strength on the separation of biologically important polar compounds: adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine triphosphate (ATP), cyclic adenosine monophosphate (cAMP), creatine (C) and phosphocreatine (CP) was tested. All analyses were performed using isocratic elution. The mobile phases consisted of an organic part - acetonitrile (ACN) and an aqueous part - buffer (sodium phosphate dibasic - Na2HPO4 with sodium fluoride - NaF) in different ratios. The mobile phase flow rate was at 1 mL/min. The detection of analytes was performed by using a photodiode array detector at wavelengths of 215 nm for C and CP..