The employment of HPLC in chiral separation II.

Employment of HPLC in chiral separation II. Diploma thesis Martina Bartošová Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Heyrovského 1203, Hradec Králové. The need to obtain enantiomerically pure substance increases due to the permanent growing demands on quality, efficiency and safety of drugs. The importance of chiral separations, which are necessary in the area of drug production as well as drug analysis, raises as a consenquence of this trend. There are several methods, which are capable of chiral separations. The most favourable are chromatographic methods, where the high performance liquid chromatography (HPLC) occupies the leading position. This method is being constatntly developed. High efficiency and fast separation of enantiomers are the main driving forces. The main demands are stable chiral stationary phases (CSP), which are able to achieve baseline enantiomeric separation in the shortest time as possible. The chiral stationary phases have to prove high selectivity, efficiency and mechanic resistance. This phenomenon leads to the continual search for new options in the area of CSPs. New alternatives have been in chiral selectors, which are responsible for the nature of chiral separations, and also in..