Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Anna Drbohlavová Supervisor: Mgr. Marcela Vejsová, Ph.D. Diploma thesis title: Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method Objective The occurrence of resistant bacteria strains is the global problem today, which influences everyone's life. One of the way, how to solve this problem, is to develop new effective antibacterial substances. The goal of the diploma thesis was to test 70 potencial medical drugs and find out their antibacterial activity against selected bacteria strains. Methods The minimal inhibitory concentrations of tested substances, affecting the growth of the most common 8 conditionally pathogenic bacteria, including significant resistant strains such as MRSA and Klebsiella pneumoniae producing extended specter beta lactamase, were monitored by microdilution broth method. Results Tested substances were divided into eight groups according to their chemical structure and then their antibacterial effect was compared. The most effective group was presented by salicylanilide and its derivates. The influence of the chemical structure and production of clot in growth medium of these substances on..