Modulatory effects of apigenin and luteolin on cytochrome P4501A expression and activity in intestinal cells

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Barbora Šalovská Title, Name, Surname of tutor: Doc. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: Modulation effects of apigenin and luteolin on activity and expression of cytochrome P4501A2 in intestinal cells Flavonoids are a large group of polyphenolic compounds that occur naturally in plant kingdom. They have been shown to posses a variety of biological activities including anti-inflammatory, anti-oxidant and anti-mutagenic effects and play a prominent role in cancer prevention. The aim of this work was research of the effect of flavones apigenin and luteolin on an activity and expression of subfamily 1A of cytochromes P450 in human colon cancer cell line HCT-8. The CYP1A isozymes enzymatic activity was measured by EROD/MROD activity assay; the quantity of CYP1A's protein was set by Western blotting. We found out that apigenin and luteolin significantly decreased CYP1A activity in HCT-8 cells. During synchronous application of model inductor CYP1A β-naftoflavon (β-NF) with apigenin or luteolin (at the concentrations of 5µM and higher), the induction effect of β-NF on CYP1A was reduced. Apigenin and luteolin significantly decreased the CYP1A activity..