Modulatory effects of quercetin and rutin on cytochrome P4501A expression and activity in intestinal cells

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Marie Volková Supervisor: Doc. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: Modulatory effects of Quercetin and Rutin on the activity and expression of cytochrome P4501A in the intestinal cells Flavonoids originate as the secondary metabolites of plants and they are daily consumed in fruits and vegetables. They show the antioxidant, antiinflammatory, anticarcinogenic and other healthy effects. A potential anticarcinogenic effect is mediated by the strong antioxidant activity, but it can also include an inhibition of xenobiotica - metabolizing enzymes to the carcinogenic compounds. This work has examined the potential inhibition effect of the flavonoids Rutin and Quercetin on an activity and expression of cytochrome P4501A (CYP1A) in the intestinal cells. Quercetin is the most abundant flavonoid and Rutin is its most abundant glycosidic form. CYP1A is responsible for an activation of many pre - carcinogenic ingested substances. The study was conducted on the intestinal cell line HCT-8. CYP1A's enzymatic activity was measured by EROD/MROD activity; quantity of CYP1A's protein was set by Western blotting. Quercetin at the concentration of 1 µM caused slightly increased EROD/MROD..