Effect of albendazole on activity of biotransformation enzymes in lancet fluke

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, name, surname of aspirant: Aneta Hradecká Title, name, surname of supervizor: Doc. Ing. Barbora Szotáková, Ph.D. Title of diploma thesis: Effect of albendazole on activity of biotransformations enzymes in lancet fluke Disease caused by lancet flukes, dicrocoeliosis, can lead to dicrease of ruminants utility and consequently to economic loses. Albendazole (the benzimidazole anthelmentic) is one of the most important drugs being used in therapy of dicrocoeliosis. Because of the need to use high and repeated dosage of albendazole, a drug resistance can occure. The aim of present thesis was to determine the influence of albendazole on lancet fluke biotransformation enzyme activity. In this experiment, lancet flukes isolated from the liver of mouflons were used. One group of lancet flukes was incubated with albendazole, another group was used as a control sample. The enzyme activity of chosen enzymes (flavine-containing monooxygenases, carbonyl-reducing enzymes, glutathione S-transferases, UDP-glucuronosyl transferases, UDP-glucosyl transferases) was determined in cytosolic fraction, mitochondria and microsomes of both groups of parazites. In the majority of cases, albendazole did not affect the..