Analytical evaluation of the pharmaceutical formulations using chromatographic methods I

Analytical evaluation of the drugs using chromatographic methods I. (Determination of the conditions for simultaneous evaluation of clotrimazole and benzylalcohol) Thesis Michal Hladík Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Analysis Optimum chromatographic conditions for simultaneous HPLC determination of clotrimazole and benzylacohol in a pharmaceutical formulations (2 % cream) were elaborated. The best results was achieved using chromatographic column Separon SGX CN, 7 μm, 150 x 4 mm I. D. (Tessek Prague, Czech Republic). The mobile phase consisted of mixture of acetonitrile and aqueous solution of K2HPO4 0.05 mol/l (60 : 40), pH 6.7 (adjusted with 10 % H3PO4). The flow rate was 0.8 ml/min. The UV detector set at 268 nm was used. Method was validated for linearity and robustness. Complete validation will be an aim of the next rigorous work