Influence of agronomical practices and plant biological protection on production of secondary metabolites of Papaver somniferum II.

Cincibuchová, V.: Influence of agronomical practices and plant biological protection on production of secondary metabolites of Papaver somniferum II. Diploma thesis. Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2013, 97 pp. The influence of agronomical practices as well as the use of biological plant protection on the production of major secondary metabolites, alkaloids morphine, codeine, papaverine, thebaine and narcotine was studied in four different varieties of the opium poppy, Papaver somniferum L. In the experiment, the seeds of the Lazur, Orbis, Orfeus and OP-P-13 varieties were used, and Pythium oligandrum, the effective microorganism of the fungicide preparation Polyversum, was applied as a biological protection. Each variety was grown in three repetitions using the application of the biofungicide and in three separate repetitions without the use of the biofungicide. Alkaloids were isolated from the poppy through the process of extraction and then analyzed using high performance liquid chromatography (HPLC). The biosynthesis of alkaloids was affected by many external factors, for example, drought and above average temperatures during the vegetation period of the poppy. The biological protection did not..