Changes of the catecholamins, serotonine and lactate levels during sport climbing on a climbing wall depending on the leader climber's style of belaying

Title: Changes of the catecholamins, serotonine and lactate levels during sport climbing on a climbing wall depending on the leader climber's style of belaying. Goals: The Goal of this thesis is to build on the bachelor thesis and develop its goals. The research is focused on a detection of blood hormonal changes before performance, right after it and after a 15- minute pacification depending on a style of a personal profile and style of belaying of the climbers. Hormonal changes have been compared during the performance on two climbing routes of the same difficulty and different styles of belaying. Methods: The randomized research sample consisted of 10 women, climbers, who climbed two routes using the OS (on sight) style on the level of maximum effort. One of the routes was climbed with clipping in protection points against the second route, which was climbed without protection points. The research was performed using a blood collection from v. brachialis and spinning off the blood plasma of the collected blood. Lactate was determined on Siemens Adria 1800 in a routine laboratory, serotonine was determined using LS-MS/MS with a gradient elution. Catecholamines were determined using LS-MS/MS with an isocratic elution. The survey composed of three psychological questionnaires (DMV, RCAI, Eysenck's..