Testing antioxidant activity using luminol chemiluminescence system

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Gabriela Chládková Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Consultant: Doc. RNDr. Miroslav Polášek, Csc. Title of Diploma Thesis: Testing antioxidant activity using luminol chemiluminescence systém The aim of the thesis was to compare various conditions for the testing of the antioxidant activity by means of the luminol chemiluminescent system in the flow system, and evaluation of dependence of the antioxidant efficacy on concentration. The antioxidant efficacy was described as dependency of the decrease of a chemiluminescence signal on the used concentration of an antioxidant. Efficacy of three substances - resveratrol, p-coumaric acid and trolox was observed. Trolox was used as a standard substance in evaluation of the antioxidant activity. The chemiluminescence system was based on a chemiluminescent reagent. In this particular case we used luminol in a sodium hydroxide solution or in a sodium carbonate solution adjusted to pH 10 or without pH adjustment. Luminol demonstrated chemiluminescence emission after oxidation with hydrogen peroxide in the presence of catalysts. As the catalyst, potassium hexacyanoferrate(III) or potassium hexacyanoferrate(II) were used. The working..