Comparsion of iron reducing properties of flavonols and flavanones

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Veronika Kyjovská Supervisor: P emysl Mlad nka, Ph.D. Title of Thesis: Compare iron-reducing properties of flavanols and flavanons The aim of this thesis was to compare the iron-reducing properties of flavanons and flavonols in different concentrations, at different pathophysiological pHs and in non-buffered conditions. A spectrophotometric method with ferrozin, an indicator of ferrous ions, was used. Results at pH 4.5 and in non-buffered conditions were almost comparable, while at pH 5.5 flavonoids showed almost no reduction activity. At pH 6.8 and 7.5 flavonoids did not reduce ferric ions at all. Representatives with the highest reduction activity belonged mostly to the group of flavonols (quercetin, kaempferol, morin, myricetin). Only one representative of flavanons - taxifolin showed the same reduction activity as aforementioned representatives of flavonols. This is probably due to the presence of the 3-hydroxy group in C ring. Other representatives of flavonon do not contain this group and were associated with minimal capabilities of reduction. Lower reduction activity was also observed in case of a representantive of flavonols - rutin, which has the 3-hydroxy group substituted..