Use of capillary electrophoresis with contactless conductivity detection for the analysis of proteins

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Kateřina Vatrasová Supervisor: doc. RNDr. Miroslav Polášek, CSc. Consultant: PharmDr. Klára Petrů, Ph.D. Title of Diploma Thesis: Use of capillary electrophoresis with contactless conductivity detection for the analysis of proteins Nowadays there exist many methods for the determination of proteins. The aim of this work was to develop, optimize and characterize method for the analysis of a model mixture of proteins (α-lactalbumin, β-lactoglobulin, carboanhydrase, lysozyme and ribonuclease) by capillary electrophoresis with conductivity detection and to compare it with conventional capillary electrophoresis with UV detection. The influence of the capillary inner wall coating on the adsorption of proteins, the composition and concentration of convenient background electrolyte, and the voltage and temperature were examined as experimental conditions possibly affecting the separation. A 50-cm fused-silica capillary (effective length 35 cm for conductivity detector and 41.5 cm for UV detector) with internal diameter 50 μm was used for the analysis. The UV detection wavelength was 210 nm. The static coating of the inner capillary wall realized by successive multiple soaking with..