The alkaloids of the genus Narcissus (Amaryllidaceae) and its biological activity

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of pharmaceutical botany and ecology Candidate: Mgr. Václav Hrstka Consultant: Doc. Ing. Lucie Cahlíková, Ph.D. Title of Thesis: The alkaloids of the genus Narcissus (Amaryllidaceae) and its biological activity The content of this thesis was to prepare alkaloidal extracts from 6 selected species of genus Narcissus. Summary extracts were evaluated by GC/MS analysis for identification of each alkaloid and measuring of their inhibitory activity to human cholinesterases (HuAChE and HuBuChE) was included. The results obtained were subsequently identifying the most suitable species for more detailed analysis From tested plants the most active extract was from species Narcissus canaliculatus, IC50 = 7,28 ± 0,66 µg/ml respectively. The best inhibitory effect on both the esterase had extract from the plant Narcissus cv. Ice Follies, which reached values of IC50 = 7.28 ± 0.66 µg/ml for HuAChE and IC50 = 14,95 ± 2,01 µg/ml for HuBuChE, which is the best result of the downturn HuBuChE from tested plants. Significant activity against HuBuChE was also reflected in the extract from the cultivar Narcissus bulbocodium var. conspicus, which has been measured by IC50 = 22,37 ± 3,14 µg/ml. Keywords: Amaryllidaceae, alkaloids, Narcissus sp.,..