Analysis of drugs and potential drugs in biological material using liquid chromatography

Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Petra Reimerová Supervisor: doc. PharmDr. Petra Štěrbová, Ph.D. Title: Analysis of drugs and potential drugs in biological material using liquid chromatography Bioanalytical methods play important role during development of new drugs. This is because determining the concentration of the drug of interest in biological matrices can help to reveal its mechanism of action, determine the values of basic pharmacokinetic parameters or study the fate of a potential drug during in vitro experiments. Considering the complexity of biological samples and low concentrations of studied compounds, the sample preparation prior to analysis is really important. Miniaturized techniques derived from standard extraction methods have significant presence in modern bioanalysis, mainly because they decrease both required sample volume and consumption of laboratory material to a minimum, and they enable automatization. One of the main areas of drug discovery is currently the treatment of cancer. Unfortunately, development of highly efficacious drugs and prolongation of patient survival have resulted in the risk of developing late complications associated with anticancer treatment..