Subvulvarni leiomiosarkom u krave simentalske pasmine - prikaz slučaja

In this case, a mass located subcutaneously in the perineal subvulvar region of a six-year-old Simmental cow was examined clinically, pathomorphologically and immunohistochemically. Macroscopically, the solitary, hitishyellow- tumor mass was 19x15x6 cm in size, weighed 1610 grams, and had a lobular structure with a few small cystic formations in the section. Histopathological examination revealed that the tumor parenchyma had smooth muscle-like cells with abundant cytoplasm, pleomorphic cells with blunt-ended or cigar-shaped nuclei, anisocytosis, anisokaryosis and karyomegaly. Immunohistochemically, strong positive expression for α-SMA, vimentin, Ki67 and slight positive for desmin were found, while immulolabeling for pancytokeratin (AE1/AE3), S-100, CD31 and CD34 were negative. In conclusion, on the basis of these findings, the tumor was diagnosed as leiomyosarcoma.U šest godina stare krave simentalske pasmine klinički, patomorfološki i imunohistokemijski obrađena je tvorba smještena supkutano u perinealnoj subvulvarnoj regiji. Solitarna, žutobjelkasta tumorska tvorba bila veličine 19 x 15 x 6 cm i težila je 1610 g. U režnjevitoj građi uočeno je nekoliko manjih cističnih formacija. Histopatološka pretraga pokazala je da tumor parenhima ima stanice nalik na glatke mišićne stanice s obilnom citoplazmom i pleomorfne stanice sa zaobljenom jezgrom ili jezgrom u obliku cigare, te s anizocitozom, anizokariozom i kariomegalijom. Imunohistokemijski je pronađena jaka pozitivna ekspresija za α-SMA, vimentin, Ki67 i slabo pozitivna za dezmin, dok je imunoobilježavanje za pancitokeratin (AE1/AE3), S-100, CD31 i CD34 bilo negativno. Na temelju ovih rezultata zaključujemo da se radi o tumoru leiomiosarkomu