A genetically encoded near-infrared fluorescent calcium ion indicator
- Qian, Yong
- Piatkevich, Kiryl D.
- Mc Larney, Benedict
- Abdelfattah, Ahmed S.
- Mehta, Sohum
- Murdock, Mitchell H.
- Gottschalk, Sven
- Molina, Rosana S.
- Zhang, Wei
- Chen, Yingche
- Wu, Jiahui
- Drobizhev, Mikhail
- Hughes, Thomas E.
- Zhang, Jin
- Schreiter, Eric R.
- Shoham, Shy
- Razansky, Daniel
- Boyden, Edward S.
- Campbell, Robert E.
Publication date
November 2021
DOI Publisher
Springer Science and Business Media LLC Journal
Nature MethodsAbstract
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators
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