Swimming kinematics and performance of spinal transected lampreys with different levels of axon regeneration

© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Fies, J., Gemmell, B. J., Fogerson, S. M., Morgan, J. R., Tytell, E. D., & Colin, S. P. Swimming kinematics and performance of spinal transected lampreys with different levels of axon regeneration. Journal of Experimental Biology, 224(21), (2021): jeb242639, https://doi.org/10.1242/jeb.242639.Axon regeneration is critical for restoring neural function after spinal cord injury. This has prompted a series of studies on the neural and functional recovery of lampreys after spinal cord transection. Despite this, there are still many basic questions remaining about how much functional recovery depends on axon regeneration. Our goal was to examine how swimming performance is related to degree of axon regeneration in lampreys recovering from spinal cord transection by quantifying the relationship between swimming performance and percent axon regeneration of transected lampreys after 11 weeks of recovery. We found that while swimming speeds varied, they did not relate to percent axon regeneration. In fact, swimming speeds were highly variable within individuals, meaning that most individuals could swim at both moderate and slow speeds, regardless of percent axon regeneration. However, none of the transected individuals were able to swim as fast as the control lampreys. To swim fast, control lampreys generated high amplitude body waves with long wavelengths. Transected lampreys generated body waves with lower amplitude and shorter wavelengths than controls, and to compensate, transected lampreys increased their wave frequencies to swim faster. As a result, transected lampreys had significantly higher frequencies than control lampreys at comparable swimming velocities. These data suggest that the control lampreys swam more efficiently than transected lampreys. In conclusion, there appears to be a minimal recovery threshold in terms of percent axon regeneration required for lampreys to be capable of swimming; however, there also seems to be a limit to how much they can behaviorally recover.This research was funded by a National Science Foundation CBET award to S.P.C. (2100156) and B.J.G. (2100703) and by Marine Biological Laboratory institutional funds awarded to J.R.M. and provided by the Eugene Bell Center Endowment, Rowe Endowment for Regenerative Biology, and Charles Evans Research Development award. Deposited in PMC for immediate release