Effects of various agitation techniques on sealer penetration in dentinal tubules using CLSM: An in-vitro study

Aim: To compare the effect of ultrasonic, sonic and rotary sealer activation techniques on depth of penetration of sealer in the dentinal tubules. Materials and Methods: Freshly extracted 60 single rooted human permanent mandibuar premolar with mature apex were collected. A size 10 no. K file (Dentsply Maillefer) was introduced into each canal. The irrigation procedure was accomplished by using 2ml of 5.25% NAOCl for each file used. Roots were randomly divided into 3 groups on the basis of sealer activation techniques. The specimens were kept in incubator at 37 degree Centigrade temperature for 2 days. The dentin segments were examined on a confocal microscope in Jawaharlal Nehru University, New Delhi under 4X,10X magnification. Results: A total of 60 teeth were included in the study and they were divided into three groups on the basis of method of agitation of sealer: GROUP I - Lentulospiral, Group II-Ultrasonic endodontic tip, Group III - Endoactivator and obturation was done with gutta percha. Mean percentage and depth of penetration was greatest in Group II i.e. Ultrasonics when used for agitation and statistically significant than Group I lentulospiral and Group III Endoactivator.&nbsp