Phenotypic and genotypic study of some virulence factors for main bacteria which caused to UPEC

The study was aim to isolating and identification of bacteria from urinary tract infection. The present study includes 240 samples collected from patients suffering from urinary tract infection during the period extended from September 2021 to December 2021 (female and male ). From 240 specimens 160 (66.6%) samples were females and 80 (33.3 %) were males .Out of the total 240 specimens only 195 (81.3%) specimens showed significant bacterial growth ,135(69.2%) of female specimens and 60 (30.7%) from male specimens. The result of our study found that the many bacterial species are responsible for causes urinary tract infection , they have been included 9 bacterial species and the most common pathogen was Escherichia coli 85 (43.5%) , Klepsiella pneumonia 32 (16.4%) ,Enterobacter19(9.7%), S. aureus 20(10.2%) , S. sarophyticus 10 (5.1%) Proteus mirabilis 9 (4.6%), Pseudomonas aeruginosa 9 (4.6%), Citrobacter koseri 9(4.6%) and Salmonella typhi 2 (1%). In this study, some of virulence factor were detected by phenotypic methods while others detected by phenotypic and then genotypically by using PCR techniques. The phenotepic investigated include biofilm formation, capsule produced, hemolysis and enzymes which were protease, DNase, and gelatinase.&nbsp