Survey the bacterial types in breast tissue and CA13-5 for women with breast disease in Babylon province

Breast tumor may be malignant and benign so these tumor and breast diseases are harboring  bacteria  ,but types of bacteria may differ  among  breast tumors so the current study was aimed to detect type of bacteria in breast tissue .  Specimens(  Breast tissue and blood) was collected from 50 female (aged ranged between 14 to 60 ) undergoing breast surgery at AL-Hilla Teaching Hospital and Al-Sadiq Hospital in Babylon Province during   period from November 2021 - March 2022 and blood sample also collected from healthy women as control  . tissue obtained was combined  outside the marginal zone, approximately 5 cm away from the neoplasm. After enucleation, the new tissue was immediately placed in a sterile plane tube or urine cup  it contain a normal saline solution  and it was cut , homogenized by using a sterile surgical scalpel and wooden sticks  within 30 min of collection.2 it was cultivated on blood agar &MacConkey media  ,and then on selective media .and Identification for bacteria by microscopic ,biochemical test and confirmed by Vitec 2Compact System .  The results appeared the percentage of malignant tumor (17.14%) and benign (82.85%) , The growth percentage (88.57%) while no growth was  (11.42%)