Development and validation of UV spectrophotometric method for trimethoprim in pure and marketed formulation

Trimethoprim drug is an aminopyrimidine antibiotic with a methylene bridge linking the pyrimidine 2,4-diamine and 1,2,3-trimethoxybenzene functional groups.It is helpful in the treatment of UTI (Urinary Tract infection). A rapid and sensitive method was develop for the analysis of Trimethoprim in both pure and tablet dosage form. The Methanolic solution of trimethoprim  pure drug  at room temperature (25 ± 10C) was absorbed at a maximum of 285 nm. Under optimal and validated test conditions ,Beer's rule was obeyed in the 10 – 60 μg / ml concentration range. The experiment was conducted for linearity, accuracy, precision(Intra day & interday precision), Range, detection limits, quantification limits, robustness, and ruggedness. The trimethoprim  was also analysed by preformulation parameters  with respect to Melting point, solubility,determination of λmax, calibration curve .The assay of marketed formulation was also performed