Antiangiogenic activity of hydroalcoholic flowers extract of Lantana Camara: In vitro, in ovo and in vivo evaluation

In current research, Lanata camara, flower hydroalcoholic extract; was evaluated for anti-angiogenic properties. The anti-angiogenic potency was evaluate by chick chorioallantoic membrane (CCAM) (in ovo), endothelial cell proliferation (ECP), transwell migration (TMA) and Matrigel cord-like morphogenesis (MCLM) assay (in vitro) along with sponge implantation method(SIM) (in vivo). The results compared to standard anti-angiogenic drug bevacizumab (BEV) and also with the angiogenic factor vascular endothelial growth factor at 500 nM concentration. In CCAM test results, the quantity of branching points(BPS) and angiogenic score(ANS) were found significant at 10-5 M and 10-4 M. Significant reductions in mass of sponge, quantity of blood vessels produced, and hemoglobin content(HB)wasfound at various concentrations of Lanata camara flower hydroalcoholic extract in SIM method. The results of human umbilical vein endothelial cells (HUVEC) the test samples (1-100 nM) displayed major inhibition of proliferation and migration and a decline in network length of cord-like tubes (CLT) in a dosage-dependent pattern. Lanata Camara, flower hydroalcoholic extract has significant anti-angiogenic activity by inhibition of cell proliferation, migration, and CLTdevelopment, that results from blocking of the cell proliferation on endothelial cells.&nbsp