Effectiveness of magnetic field in stimulation of biochemical and enzymes activities in seedling and callus of Nigella sativa

This current study investigated the influence of Magnetic Field from Winding coil magnet about ( 400 mT ) for different exposure periods ( 0, 30 , 60 , 90 , 120 , 150 min ) on seed germination, callus initiation, and callus fresh weight, protein, carbohydrate and proline content and vitality , in addition to the efficacy of some antioxidant enzymes . The results indicated that the highest seed germination percentage was when exposed for an (90 Minutes). Also found that the best exposure period for acceleration of callus initiation was (30 minutes). Fresh weight, protein, proline, carbohydrates content and callus vitality got a better result under (90 minutes) of magnetic field exposure . On the other hand, the antioxidant enzymes activity significantly increased with 150 minutes M.F treatment