The protective effect of flagellin A and B as candidate vaccine against Pseudomonas aeruginosa respiratory infections

Background: Multi drug resistance (MDR) P. aeruginosa consider the main cause of morbidity in hospitalize patients suffering from chronic airway infections such as chronic pulmonary disease, pneumonia and cystic fibrosis (CF). Also, the rapidly development of antibiotic resistance to the last generations of a broad spectrum antibiotic were detected for many P. aeruginosa isolates. Accordingly the vaccines have the potential to prevent and treatment such infections. Aim: The present study aimed to investigate the active immunization using flagellin to provide the protection against MDR P. aeruginosa clinical isolates in the fatal respiratory acute infection in animal model. Methods: Flagellin a and flagellin b purified partially from local isolates of P. aeruginosa were used for animal immunization. After that the animals were immunized intranasally after sedative the animal by applying 10 μl of flagellin a (4.8 μg), or flagellin b (4.8 μgl) on each nostril for 100 mg weight of rat at weekly intervals. After week from the 6th dose, the animal were exposed to 2*107 CFU of P. aeruginosa direct into each nostril (intranasally)