Hematological, biochemical, and histopathological changes resulting from administration of three different concentration of diazepam in mice

Diazepam is a benzodiazepine derivative that is commonly used for its sedative and anxiolytic activities. The liver plays a major role in the metabolism of this drug. By using the experimental animals, female white mice aged 2-3 months were divided into four groups. Each group contains three mice. The first group (the control) was given distilled water for four weeks. While the second, third and fourth groups were given diazepam at concentrations (0.25 mg / ml, 0.50 mg / ml, 0.75 mg / ml), respectively, for four weeks, orally  using stomach tubes. Blood was drawn, to obtain the blood for hematological tests and the serum for biochemical tests. Rats were dissected, and samples were taken to the laboratory for the purpose of analyzes and tissue segmentation of the liver, spleen. Liver and spleen tissues were obtained for the histopathology analysis and serum for the assay of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and Alkaline phosphatase (ALP)