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Measurement of 4-Hydroxynonenal (4-HNE) Protein Adducts by ELISA

Mehta, K.
Patel, V.B.
Mehta, K.
Patel, V.B.

Publication date

January 2019

DOI

10.1007/978-1-4939-9463-2_4.

Publisher

Springer Science and Business Media LLC

Abstract

Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens and antibodies alike. We describe here procedures, indirect ELISA and sandwich ELISA for the detection of 4-hydroxynonenal protein adducts. These adducts are stable compounds formed within cells and bodily fluids under conditions of oxidative stress. They can act as sensitive biomarkers of oxidative stress and are directly linked to disease pathology

Extracted data

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Griffiths, Helen R.
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Milkovic, Lidija
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Osmekhina, Ekaterina
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June 2010

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M.K. Chung
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April 2013

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Enzyme immunoassay for a urinary metabolite of 4-hydroxynonenal as a marker of lipid peroxidation

Guéraud, Françoise
Peiro, Géraldine
Bernard, Herve,
Alary, Jacques
Créminon, Christophe
Debrauwer, Laurent
Rathahao, Estelle
Drumare, Marie-Francoise
Canlet, Cécile
Wal, Jean-Michel,
Bories, Georges

January 2006

International audienceFree radical reactions are involved in the pathogenesis of numerous diseases, ...

Enzyme immunoassay for a urinary metabolite of 4-hydroxynonenal as a marker of lipid peroxidation

Guéraud, Françoise
Peiro, Géraldine
Bernard, Herve,
Alary, Jacques
Créminon, Christophe
Debrauwer, Laurent
Rathahao, Estelle
Drumare, Marie-Francoise
Canlet, Cécile
Wal, Jean-Michel,
Bories, Georges

January 2006

International audienceFree radical reactions are involved in the pathogenesis of numerous diseases, ...

Enzyme immunoassay for a urinary metabolite of 4-hydroxynonenal as a marker of lipid peroxidation

Guéraud, Françoise
Peiro, Géraldine
Bernard, Herve, H.
Alary, Jacques
Créminon, Christophe
Debrauwer, Laurent
Rathahao, Estelle
Drumare, Marie-Francoise
Canlet, Cécile
Wal, Jean-Michel, J.-M.
Bories, Georges

January 2006

International audienceFree radical reactions are involved in the pathogenesis of numerous diseases, ...