Endothelial Morphometry by Image Analysis of Corneas Organ Cultured at 31 degrees C

International audiencePurpose. To determine the factors influencing endothelial morphometry by image analysis of corneas stored in organ culture: coefficient of variation (CV) in cell area and percentage of hexagonal cells. Methods. The endothelium of 505 of the 559 corneas consecutively stored at the eye bank was routinely analyzed using the Sambacornea analyzer (Tribvn) on three large-field images of 750x1000 µm, obtained after osmotic dilation of the intercellular spaces with 0.9% sodium chloride. Analysis was done on at least 300 cells. The quality of the three-image set was graded 'poor', 'average' or 'good' by an independent observer. The studied parameters were: donor age and gender, lens status, storage time, intrinsic quality of captured images. Statistics: non-parametric tests. Results. Image analysis was possible with 504 of the 505 assessed corneas. Donor age was significantly correlated with endothelial cell density (r=-0343), CV (r=0.221) and hexagonality (r=-0.314) (p