Comparative study of Thickness of Wharton’s Jelly of Umbilical Cord in Normal and PIH Patients on histological basis by using special stain –PAS stain

Aim and objectives: To compare the thickness of Wharton’s jelly of umbilical cord in normal and PIH patients on histological basis by using special stain i.e. Pas stain1. To measure the thickness of Wharton’s Jelly of umbilical cord in normal and PIH patients.2. To measure the thickness of Wharton’s Jelly of umbilical in PIH patients.3. To compare between the thickness of Wharton’s Jelly of umbilical cord in normal and PIH patients. Method: The study sampled 50 normal placenta with umbilical cord and 50 placenta with umbilical cord from PIH patients are included in the study. After delivery, the umbilical cord with placenta were collected from labour room of MGM Hospital, Kalamboli, Navi Mumbai and fixed in 10% formalin solution. For Histology: Standard procedure for staining were used. Slides were prepared and stained with special stain. (PAS Stain). The thickness of wharton’s jelly was measured with micrometer eyepiece. Statistical analysis: Data is analysed by using statistical software SPSS version -16. Results:1. Special Stain (PAS Stain) was performed for all umbilical cord samples. In normal as well as PIH, we found 2 arteries and 1 vein.2. PAS stain was used to differentiate Wharton’s Jelly from tunica adventitia. The thickness was measured and it was found that Wharton’s Jelly is reduced in PIH cases as compared to normal. Conclusion: The Wharton’s Jelly is reduced in PIH cases as compared to normal