Analysis of TNFα promoter SNPs and the risk of cervical cancer in urban populations of Posadas (Misiones, Argentina)

Fil: Badano, Ines. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina.Fil: Stietz, Silvina M. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina.Fil: Schurr, Theodore G. University of Pennsylvania. Department of Anthropology. Laboratory of Molecular Anthropology; Estados Unidos.Fil: Picconi, Alejandra M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virus Oncogénicos; Argentina.Fil: Fekete, Daniel. Hospital Escuela de Agudos "Dr. Ramón Madariaga" Servicio de Patología; Misiones, Argentina.Fil: Quintero, Ivana M. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina.Fil: Cabrera, Maia D. E. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina.Fil: Campos, Rodolfo H. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Virología.Fil: Liotta, Domingo J. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina.Background: Human papillomavirus (HPV) plays a central role in cervical cancer development. However, only a small fraction of infected women develop the disease. Additional risk factors, including SNPs in immune system and cytokine genes, are likely to be important determinants. Objective: We investigated the potential role of cytokine TNF-α promoter SNPs (TNFα-375A, TNFα-307A, TNFα-243A, and TNFα-237A) in the development of high-grade cervical lesions and cancer in urban women from Posadas (Misiones, Argentina). Study design: Fifty-six cases (CINIII and invasive carcinoma) and 113 age-matched controls were included in the study. HPV genotype detection was conducted by PCR. TNFα SNP genotyping was conducted through PCR amplification and direct sequencing of genomic DNA. Results: We observed differences in the allelic distribution of TNFα-307A and TNFα-375A SNPs among cases and controls (p