The healing effects of hyperium perforatum (St. john’s wort) on experimental alkaline corrosive eosephageal and stomach burns [Deneysel alkali koroziv ösefageal ve mide yanıklarında hyperium perforatum’un (Sarı kantaron) iyileştirici etkisi]

BACKGROUND: The most frequent etiologic cause is alkaline substances. We investigated the protective effects of the plant St. John ‘s Wort (Hypericum perforatum). METHODS: We included 42 Wistar albino rats weighing between 200–300 grams and divided into six groups as Group 1: Con-trol, Group 2: Burn+Saline (BS), Group 3: Burn+St. John’s Wort (BSJW), Group 4: Burn+Plasebo (BP), Group 5: St. John’s Wort (SJW), Group 6: Placebo (P). After 15 days of treatment, esophagus, stomach and liver tissue samples were derived by dissection for histopathologic and biochemical markers. The cytotoxic effects of formulation on fibroblasts is evaluated in vitro on human dermoblast fibroblast line (HDFa, Gibco Invitrogen cell culture, C-013-5C). RESULTS: The weight of the rats increased in Group 1, 3, 4, 6, decreased in Group 2 and did not change in Group 5. In the BSJW group, submucosal collagen accumulation, muscularis mucosa damage, tunica muscularis damage and collagen accumulation in esophagus were similar to the control group but lesser than BS and placebo group. In the stomach, mucosal damage, gastric gland dilatation, submucosal polymorphonuclear infiltration were similar to the control group and lesser than the BS group. The lethal concentration of SJW was 2.58 gr/mL. CONCLUSION: SJW substrate is effective in protecting the esophagus and stomach in mild to moderate alcali corrosive burns in the subacute period. We should keep in mind the protective effects of STW substrate in alkaline corrosive burns of the gastrointestinal system. © 2020, Turkish Association of Trauma and Emergency Surgery. All rights reserved.Ege Üniversitesi: 2013TIP-079This experimental study is performed after the ethical approvement from Dokuz Eylul University Medicine Faculty, Animal Ethics Committee with the protocol number of 65/2013; the survey is performed according to “The Guide for the Care and Use of Laboratory Animals-Institute of Laboratory Animal Resources Commission on Life Sciences National Research Council, USA”. The experimental part of the study is carried out in Dokuz Eylul University Multidisciplinary Experimental Research and Animal Laboratory; histopathological analyses were carried out in Ege University, Faculty of Medicine Division of Histology and Embryology; biochemical analyses were carried out in Ege University Science Faculty, Department of Biology and AREL Laboratory. The study is supported by Ege University Scientific Research Project Fund as project 2013TIP-079