ABSTRACT Five moment hand hygiene is an early step in preventation of Hospital Acquired Infection (HAIs), which there are factors that can affect them, there are the knowledge and motivation. Good knowledge and great motivation be strong to hold to reduce the transmission of infection through hand hygiene and compliance to do Five moment hand hygiene. Purpose of this research was to identify the relation of knowledge and motivation nurses in compliance five moment hand hygiene at The General Hospital (RSUD) Sayang, Cianjur Regency. The research design using the layout of analytic study of the correlation with cross-sectional approach. Sampling using Probability Sampling with Proportional Stratified Random Sampling approach. The primary dat...