Rapid and sensitive HILIC-MS/MS analysis of carnitine and acetylcarnitine in biological fluids
- Isaguirre, Andrea Celeste
- Olsina, Roberto Antonio
- Martinez, Luis Dante
- Lapierre, Alicia V.
- Cerutti, Estela Soledad
DOIAbstract
Monitoring carnitine and acetylcarnitine levels in biological fluids is a powerful tool for diagnostic studies. Research has recently shown that the analysis of carnitine and related compounds in clinical samples can be accomplished by different analytical approaches. Because of the polar and ionic nature of the analytes and matrix complexity, accurate quantitation is a highly challenging task. Thus, sample processing factors, preparation/cleanup procedures, and chromatographic/ionization/detection parameters were evaluated. On the basis of the results obtained, a rapid, selective, sensitive method based on hydrophilic interaction liquid chromatography?tandem mass spectrometry for the analysis of carnitine and acetylcarnitine in serum and u...
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