Confined Gold Nanoparticles Enhance the Detection of Small Molecules in Label-Free Impedance Aptasensors
- Peinetti, Ana Sol
- Ceretti, Helena
- Mizrahi, Martin Daniel
- González, Graciela Alicia
- Ramirez, Silvana Andrea Maria
- Requejo, Felix Gregorio
- Montserrat, Javier Marcelo
- Battaglini, Fernando
DOIAbstract
A controlled architecture of nanoelectrodes, of a similar size to small molecule-binding aptamers, is synthesized inside nanoporous alumina. Gold nanoparticles with a controlled size (about 2 nm) are electrogenerated in the alumina cavities, showing a fast electron transfer process toward ferrocyanide. These uncapped nanoparticles are easily modified with a thiol-containing aptamer for label-free detection of adenosine monophosphate by electrochemical impedance spectroscopy. Our results show that the use of a limited electrical conducting surface inside an insulating environment can be very sensitive to conformational changes, introducing a new approach to the detection of small molecules, exemplified here by the direct and selective detect...
Add to ORKG