Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
- Loto, Flavia del Valle
- Romero, Cintia Mariana
- Carrizo, Alfonso Emanuel
- Baigori, Mario Domingo
- Pera, Licia Maria
DOIAbstract
A novel esterase producer strain named Bacillus sp. A60 was isolated from a soil sample contaminated with hydrocarbons. It was found to belong to Bacillus subtilis species through morphological, biochemical and 16S rRNA gene sequence analyses. This strain which can tolerate 15% (w/v) NaCl and growth at 55°C, produced an interesting esterase activity in Luria-Bertani medium. Two different molecular weight esterase activities were detected in zymographic assays. Culture supernatant and whole cells showed specific hydrolytic activities of 2.67 ± 0.11 U/mg of protein and 7.07 ± 0.09 U/mg of dry weight, respectively. Concerning ethyl acetate production, conversions of 88.00 ± 0 and 55.58 ± 0.78% were obtained with culture supernata...
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