Effects of thymol feed supplementation on female Japanese quail (Coturnix coturnix) behavioral fear response

Dietary supplementation with thymol has been shown to improve the oxidative stability of eggs and meat during storage. In addition, in vitro studies have shown that this compound can act as a positive allosteric modulator of the GABAA receptor, similarly to its analogue phenolic compound propofol. Hence, it is conceivable that thymol could also present anxyolitic and/or fear reducing properties, probably also affecting their locomotor activity. Considering that fearinducing/ stressful situations are practically unavoidable during birds´ rearing, the thymol feed supplementation could therefore present beneficial consequences in terms of animal welfare. This study evaluates potential fear reducing properties of thymol feed supplementation and its potential effects on locomotor activity by assessing female Japanese quail behavioral responses during brief mechanical restraint and openfield tests. Birds were evaluated after 2 and 15 days of supplementation. During the brief mechanical restraints, the latencies to struggle were significantly (P<0.05) shorter and the number of struggling bouts significantly higher (P