In this chapter, we describe how to conveniently demonstrate, assess, and study cell death in Dictyostelium through simple cell culture, clonogenic tests, and photonic (with the help of staining techniques) and electronic microscopy. Cell death can be convniently generated using minor modifications of the monolayer technique of Rob Kay et al., and either wild-type HMX44A Dictyostelium cells or the corresponding atg1- autophagy gene mutant cells. Methods to follow cell death qualitatively and quantitatively facilitate detailed studies of vacuolar death in wild-type cells and of nonvacuolar, "condensed" death in atg1- mutant cells
International audienceAutophagic cell death in Dictyostelium can be dissociated into a starvation-in...
Cell death is essential for a plethora of physiological processes, and its deregulation characterize...
International audienceCell death is essential for a plethora of physiological processes, and its der...
Among unusual models to study cell death mechanisms, the protist Dictyostelium is remarkable because...
Abstract We have studied the correlates of cell death during stalk cell differentiation in Dictyoste...
International audienceAutophagic cell death (ACD) can be operationally described as cell death with ...
International audienceThree main advantages make Dictyostelium a very favorable model to study the i...
International audienceDictyostelium cells in monolayers in vitro lend themselves well to a study of ...
AbstractAutophagic cell death (ACD) can be operationally described as cell death with an autophagic ...
Des conditions de carence entrainent une mort cellulaire développementale chez le protiste Dictyoste...
Dictyostelium constitutes a genetically tractable model for the analysis of autophagic cell death (A...
While necrotic cell death is attracting considerable interest, its molecular bases are still poorly ...
While necrotic cell death is attracting considerable interest, its molecular bases are still poorly ...
International audienceAutophagic cell death in Dictyostelium can be dissociated into a starvation-in...
Cell death is essential for a plethora of physiological processes, and its deregulation characterize...
International audienceCell death is essential for a plethora of physiological processes, and its der...
Among unusual models to study cell death mechanisms, the protist Dictyostelium is remarkable because...
Abstract We have studied the correlates of cell death during stalk cell differentiation in Dictyoste...
International audienceAutophagic cell death (ACD) can be operationally described as cell death with ...
International audienceThree main advantages make Dictyostelium a very favorable model to study the i...
International audienceDictyostelium cells in monolayers in vitro lend themselves well to a study of ...
AbstractAutophagic cell death (ACD) can be operationally described as cell death with an autophagic ...
Des conditions de carence entrainent une mort cellulaire développementale chez le protiste Dictyoste...
Dictyostelium constitutes a genetically tractable model for the analysis of autophagic cell death (A...
While necrotic cell death is attracting considerable interest, its molecular bases are still poorly ...
While necrotic cell death is attracting considerable interest, its molecular bases are still poorly ...
International audienceAutophagic cell death in Dictyostelium can be dissociated into a starvation-in...
Cell death is essential for a plethora of physiological processes, and its deregulation characterize...
International audienceCell death is essential for a plethora of physiological processes, and its der...