In Vitro Study of the Effect of Nanoparticles on DNA deletion/mutations

Nanoparticles are widely used in a variety of industries ranging from beauty care products to videotape production, and their influence is increasing. Nanoparticles are easily inhaled into the lungs due to their extremely small size (less than 100 nm). Once inside the lungs, their influence is compounded due to their natural tendency to form large aggregates, which allows them to remain in the lungs for a longer period of time. Some preliminary data have demonstrated that these nanoparticles were cytotoxic. However, the mechanism for the toxicity is not yet clear. To study one possible cytotoxicity mechanism of nanoparticles, DNA was incubated with varying concentrations of three different sized nanoparticles, Fe2O3, CeO2, and Ti O2. The DNA fragments were separated by capillary gel electrophoresis to determine the amount of DNA deletion/mutations caused by each