Study of Effect of Physiochemical Parameters on the Reproducibility (validation) of Biodegradation of Textile Dyes

Background: Environmental pollution due to different industrial waste has become a major problem in modern world because of their high toxic in nature. Textile wastes are carcinogenic and mutagenic contain varieties of toxic dyes and fixers. Azo dyes widely used in textile, paper and printing industries are not easily degradable and having carcinogenic nature. Due to health hazards of textile wastes, several chemical and biological methods are being used to make the discharged wastewater less toxic by degrading colors and other harmful chemicals. Present research study was aimed to evaluate the sensitivity of biodegradation process in form of Chemical oxygen demand (COD) and, to check the reproducibility of biodegradation results for validation purpose.Method: Initially selective bacterial strains were isolated from different waste samples. COD of the three samples of dyes were performed by titration method to observe the pattern of reproducibility of the biodegradability (COD) results.Results: Textile industry sludge wastewater samples contained different characteristics of dye degrading consortium of bacterial strains as compared to soil extracts samples whereas did not get any bacterial growth in domestic wastewater samples. It was interesting to observe that the reproducibility of COD results was very hard to get due to sensitivity of the test parameter to the prevailing environmental conditions.Conclusion: Present study is significant for understanding the sensitivity of reproducibility or validation of biodegradation study. On the basis of present findings of experiments, we can say that only the method of determining COD could be standardized which will ensure the credibility of the results and the changed environmental temperature could affect the controlled experimental results. Present finding would give benefit to the new scientists and students for understanding the normal behavior of biodegradation study.Keywords: Biodegradation, Bioremediation, Environmental Health, Sludge, Diseases