Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022

Claro, Ingra Morales
Romano, Camila Malta
Candido, Darlan da Silva
Lima, Evelyn Lepka de
Lindoso, José Angelo Lauletta
Ramundo, Mariana Severo
Moreira, Filipe Romero Rebello
Barra, Luiz Alberto Costa
Borges, Luciana Marques Sansão
Medeiros, Lucas Alberto
Tomishige, Marcia Y. S.
Moutinho, Tomas
Silva, Anderson José Dias da
Rodrigues, Camila Cristina Martini
Azevedo, Luiz Cesar Fernandes de
Villas-Boas, Lucy Santos
Silva, Camila Alves Maia da
Coletti, Thaís Moura
Manuli, Erika R.
O’Toole, Aine
Quick, Joshua
Loman, Nicholas
Rambaut, Andrew
Faria, Nuno R.
Figueiredo-Mello, Claudia
Sabino, Ester Cerdeira

Open PDF

Open link

Publication date

June 2022

Publisher

Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo

Language

English

Abstract

Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation