Construction of a Gene Library for Streptomyces spp. 5A

A method for fractionating genomic DNA from Streptomyces spp. 5A DNA into 6.6 to 23 kilobase (kb) fragments was established using a BamHI restriction digest. Construction of a gene library with BamHI digested Streptomyces spp. 5A DNA was attempted using Lucigen®\u27s BigEasy™ Linear Cloning Kit. However, only one in ten transformants tested proved to be true recombinants while the remaining nine were non-recombinants containing religated vector. The high background of non-recombinants was undesirable. However, the fact that at least one recombinant was found suggests that this cloning method might be optimized to achieve higher proportions of recombinants