In Vitro Shoot Multiplication of Garlic (Allium Sativum L.)

Shoot apex of Garlic, as explants, was used for experiment of bulb formation and shoot multiplication. HgCl2 (0.1%) as surface sterilizing agent was exposed five and ten minutes time duration. GA3 were added to MS basal medium and different concentration of full strength MS medium, half strength MS, one fourth MS and three fourth MS were taken to study their response on explants growth and development. In apex explants, best and early response (76%) for bulb formation and multiplication of shoots were observed in half strength MS medium containing 0.1µM GA3. while in three fourth MS medium without GA3, the bulb formation was delayed and minimum (60%)