Single Channel Simultaneous Analysis of DNA and MicroRNA

After attending this presentation, attendees will be aware of a new proposed protocol that will analyse DNA and miRNA simultaneously. This presentation will impact the forensic science community by exhibiting a new protocol that will expand the capability of forensic science laboratories with minimal modifications and expenses. It is often necessary to establish the identity of the body fluid from which a DNA profile was obtained. This issue is frequently encountered in sexual offences where it can be necessary to distinguish between saliva and vaginal material or where there is a very small amount of the body fluid present, such as trace amounts of blood on a dark surface. This issue is currently being resolved by the use of mRNA and microRNA (miRNA) analysis utilising a co-isolation technique to separate out the DNA and the miRNA phases for subsequent analysis