International audienceSaccharomices cerevisiae (yeast iso-1) cytochrome c has been investigated in the presence of 100 mM SDS in order to simulate the interaction of cytochrome c with membrane. Under these circumstances, a high spin species with detached methionine axial ligand is observed through NMR, in analogy to findings on the horse heart protein. However, at variance with the latter system, for the yeast protein also a low spin species is detected, which appears to be present with a concentration of about 40% with respect to that of the high spin species. The R(1), R(2), [1H]-15N NOE of backbone amides which are not affected by paramagnetism are homogeneous and allow a simultaneous analysis of the data for the two species. The result ...
Nonnative states of proteins are involved in a variety of cellular processes, including translocatio...
This thesis is a study of the structure of horse-heart cytochrome-c, and the relationship of its str...
An (15)N-enriched sample of the yeast iso-1-ferricytochrome c triple variant (Lys72Ala/Lys79Ala/Cys1...
International audienceSaccharomices cerevisiae (yeast iso-1) cytochrome c has been investigated in t...
Conformational change of cytochrome c (cyt c) caused by interaction with cardiolipin (CL) is an impo...
The effect of glycerol on the structure of cytochrome c was investigated by circular dichroism, abso...
The influence of cytochrome c binding to cardiolipin bilayers on the motional characteristics of eac...
31P NMR measurements were conducted to determine the structural and chemical environment of beef hea...
Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed...
We have employed fluorescence energy transfer (FET) kinetics to probe unfolded and molten globule st...
The ^1H NMR spectrum of the the cyanide adduct of a triply mutated Saccharomyces cerevisiae iso-1-cy...
A cysteine-specific methanethiosulfonate spin label was introduced into yeast iso-1-cytochrome c at ...
AbstractBackground: Cytochrome c has five distinct pH-dependent conformational states, including two...
His26Tyr and His33Tyr mutants were obtained from the Cys102Thr variant of yeast iso-1-cytochrome c. ...
At high concentration (98% or higher, v/v), glycerol induces collapse of acid-denatured cytochrome c...
Nonnative states of proteins are involved in a variety of cellular processes, including translocatio...
This thesis is a study of the structure of horse-heart cytochrome-c, and the relationship of its str...
An (15)N-enriched sample of the yeast iso-1-ferricytochrome c triple variant (Lys72Ala/Lys79Ala/Cys1...
International audienceSaccharomices cerevisiae (yeast iso-1) cytochrome c has been investigated in t...
Conformational change of cytochrome c (cyt c) caused by interaction with cardiolipin (CL) is an impo...
The effect of glycerol on the structure of cytochrome c was investigated by circular dichroism, abso...
The influence of cytochrome c binding to cardiolipin bilayers on the motional characteristics of eac...
31P NMR measurements were conducted to determine the structural and chemical environment of beef hea...
Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed...
We have employed fluorescence energy transfer (FET) kinetics to probe unfolded and molten globule st...
The ^1H NMR spectrum of the the cyanide adduct of a triply mutated Saccharomyces cerevisiae iso-1-cy...
A cysteine-specific methanethiosulfonate spin label was introduced into yeast iso-1-cytochrome c at ...
AbstractBackground: Cytochrome c has five distinct pH-dependent conformational states, including two...
His26Tyr and His33Tyr mutants were obtained from the Cys102Thr variant of yeast iso-1-cytochrome c. ...
At high concentration (98% or higher, v/v), glycerol induces collapse of acid-denatured cytochrome c...
Nonnative states of proteins are involved in a variety of cellular processes, including translocatio...
This thesis is a study of the structure of horse-heart cytochrome-c, and the relationship of its str...
An (15)N-enriched sample of the yeast iso-1-ferricytochrome c triple variant (Lys72Ala/Lys79Ala/Cys1...