Does urbanization impact terrestrial vertebrate ectotherms across a biodiversity hotspot?

Urbanization is increasing at an alarming rate altering biodiversity. As urban areas sprawl, it is vital to understand the effects of urbanization on biodiversity. Florida is ideal for this research; it has many reptile species and has experienced multiple anthropogenic impacts. Herein, we aim to evaluate human impacts on registered reptile richness across an urbanization gradient in Florida. The expectation is that highly urbanized areas would harbor a lower number of species. To represent urbanization, we used Venter et al. (2016) human footprint index. We downloaded georeferenced occurrence records from the Global Biodiversity Information Facility to collate species richness. We ran generalized linear regressions controlling for spatial autocorrelation structure to test the association between urbanization and reptile records across Florida. We found a positive association between urbanization and registered reptiles across Florida for total and non-native species richness; however, a lack of association occurred for native species. We performed rarefaction curves due to an inherent bias of citizen science data. The positive association was supported for non-native reptile species richness with greater species richness located at urban centers. Interestingly, total and native species richness were largest at low as well as moderate levels of urbanization. Thus, moderately urbanized areas may have the potential to harbor a similar number of reptile species compared to areas with low urbanization. Nevertheless, a difference exists in sample completeness between the urbanization categories. Thus, a more systematic monitoring of reptile species across an urbanization gradient, not only focusing on urban and wild areas but also including moderate levels of urbanization, is needed to provide informed conservation strategies for urban development planning. Advances in environmental sensors, environmental DNA, and citizen science outreach are necessary to implement if we are to effectively monitor biodiversity at the accelerated rate of urbanization