Major groundwater flow routes in the Barton Springs segment of the Edwards Aquifer closely follow the structural trends of the Balcones Fault Zone. Locations of these groundwater flow routes and velocities of groundwater flowing along the routes were determined by injecting fluorescent, non-toxic, organic dyes (Fluorescein and Eosine) into two caves within Onion Creek, the largest capacity losing stream in the Barton Springs segment of the Edwards Aquifer. Cripple Crawfish and Antioch caves are located about 17.5 mi and 14.0 mi south of Barton Springs, the primary discharge point from the aquifer, respectively. First arrival of dyes from each of these caves to Barton Springs occurred after about 3.5 and 7.0 days, indicating minimum groundwa...