Isolation, identification and cytotoxic activity of secondary metabolites from fruiting bodies of Fomitopsis betulina and application of HR MAS NMR spectroscopic technique in determination of metabolic profile

Predmet proučavanja ove doktorske disertacije je medicinska gljiva Fomitopsis betulina poznata kao brezova guba ili brezov trud. Osnovni cilj ove disertacije je bio izolovanje bioaktivnih jedinjenja triterpenskog tipa i ispitivanje citotoksičnosti na ćelijskim linijama akutne promijelocitne leukemije (HL-60) i adenokarcinoma pluća (A549), kao i DNK protektivne aktivnosti na humanim limfocitima periferne krvi. Drugi deo disertacije odnosio se na kultivaciju plodonosnih tela u laboratorijskim uslovima i dobijanje metabolomičkih modela primenom rezultata FTIR i HR MAS NMR spektroskopskih tehnika za praćenje promena u hemijskom sastavu. U okviru ove disertacije iz plodonosnih tela nekultivisane nativne gljive izolovano je i okarakterisano 31 jedinjenje triterpenskog tipa, od toga 13 jedinjenja (19-31) je po prvi put opisano u literaturi...The main goal of this dissertation was to isolate and characterize bioactive triterpene compounds from the medicinal mushroom Fomitopsis betulina and to investigate their cytotoxic activity against HL-60 leukemia and A549 lung cancer cell lines and DNA protective activity on peripheral human lymphocytes. Another aim was to employ FTIR and HR MAS NMR spectroscopic techniques in combination with metabolomics models to monitor changes in chemical composition. Within this dissertation, 31 triterpene type compounds were isolated and characterized from native fruiting bodies collected, while 13 (19-31) compounds were described in the literature for the first time..