3D Bioprinted Structures from Cells of Non-Epithelial Mesodermal and Endodermal Lineage Using a Custom Accessible 3D Bioprinting Platform

Prior work within our lab has demonstrated the ability to print both murine and human mammary organoids and tumoroids in vitro that can also be reliably transplanted into a murine host for translational studies. Peripherally, this bioprinting system has also been used for 3D printing neurons, stem cells, cancer cells, and a primary cell line rich with fibroblasts, but each of these efforts were with cells of ectodermal lineage. Thus, the system\u27s capacity for use on cells of other origins had been untested. To address this, we have now developed protocols for cells of endodermal and non-epithelial mesodermal/mesenchymal lineage. In this work, we find that we can produce reliable organoids, tumoroids, and other in vitro structures from them, thus expanding the functional range of our open 3D bioprinting platform. Therefore, we demonstrate that our system is versatile for adaptation to multiple cellular systems and can be applied to the work of labs that wish to study development and pathologies in other organ systemshttps://digitalcommons.odu.edu/gradposters2022_engineering/1001/thumbnail.jp