Investigating the Role of the Nervous System in Limb Regeneration: A Proteomics Analysis

Regeneration is a biological ability belonging to a small subset of vertebrates including the axolotl (i.e., Ambystoma mexicanum), an amphibian that can regenerate its limbs as well as other organs such as the heart and the brain, throughout its lifespan. While it is known that the nerves play a critical role in promoting limb regeneration, the potential implication of other nervous system components is largely unknown. Proteomics screening approaches were implemented in our lab to compare protein expression patterns at various time-points into the regeneration program following limb amputation. In the context of the current honors thesis project, we identified proteins that are upregulated and/or downregulated throughout limb regeneration program, and we are currently working towards exposing novel protein networks that are activated during this fascinating process