A Rapid Method for the Purification of RNA Polymerase Holoenzyme From Escherichia Coli

Mehrpouyan, Majid
Champney, W. Scott

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Publication date

January 1990

DOI

10.1016/0165-022X(90)90004-V

Publisher

Elsevier BV

Abstract

A method is described for the rapid purification of RNA polymerase holoenzyme from small amounts of Escherichia coli cells. Chromatography of a crude extract on a single-stranded DNA agarose column followed by gell filtration chromatography gave 95% pure holoenzyme. The enzyme kinetic characteristics on T7 DNA identical to those of RNA polymerase purified by other more laborious procedures

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A Rapid Method for the Purification of RNA Polymerase Holoenzyme From Escherichia Coli

Abstract

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A Rapid Method for the Purification of RNA Polymerase Holoenzyme From Escherichia Coli

Abstract

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