Application of Aqueous Gel Permeation Chromatography With in-Line Multi-Angle Laser Light Scattering and Differential Viscometry Detectors for the Characterization of Natural Product Carbohydrate Pharmaceuticals

Natural product complex carbohydrate polymers are currently being developed as prophylactic and/or therapeutic drugs. These water-soluble carbohydrate pharmaceuticals, which are primarily 0-1,3-D-glucan polymers, belong to the class of drugs known as biological response modifiers (BRMs). A major obstacle to the development, understanding and clinical utilization of carbohydrate BRMs has been the difficulty involved in accurately characterizing high molecular weight (MW) carbohydrate polymers. Recent advances in aqueous gel permeation chromatography (GPC), differential viscometry (DV) and multi